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NPCs express environmental sensors. (A) Z-stack confocal image (from a total of n5 40 Z-stacks of optical slices in 0.5 μm intervals) of a mouse neurosphere in vitro. Red is for vimentin, green is for CD44, white is for phosphor-histone H3 (pHH3) and blue is for cell nuclei (Dapi). (B) Volocity V®-based 3D reconstruction of the CD44 expression in A. The magnified frame in A shows a pHH3+/CD44+ NPC.
