Table 1.
Protein spot displaying intensity differences (p < 0.05) between obese children (OB) and controls (C) before FDR analysis
| Spot |
Spot intensity in OB
|
MS
|
MS
|
MS
|
MS -MS
|
MS-MS
|
MS-MS
|
Identification |
|---|---|---|---|---|---|---|---|---|
|
Significance level
|
Score/Cut-off | Matched fragments | Sequence coverage (%) | Score/Cut-off | Matched (Significant) fragments | Sequence coverage (%) | ||
| 1809 |
Upregulated, p < 0.05 |
68/66 |
15 |
26 |
45/40 |
2 (1) |
6 |
Alpha 1B acid glycoprotein |
| 2201 |
Downregulated, p < 0.01 |
115/66 |
12 |
50 |
98/41 |
9 (6) |
40 |
Apolipoprotein A1 |
| 2203 |
Downregulated, p < 0.05 |
44/66 |
3 |
-- |
102/41 |
3(3) |
6 |
Clusterin (Apolipoprotein J) |
| 2301 |
Downregulated, p < 0.05 |
56/66 |
7 |
-- |
95/41 |
5(4) |
23 |
Clusterin (Apolipoprotein J) |
| 2306 |
Upregulated, p < 0.05 |
75/66 |
7 |
30 |
71/41 |
6(2) |
17 |
Haptoglobin |
| 3201 |
Downregulated, p < 0.05 |
137/66 |
16 |
59 |
81/41 |
8(4) |
33 |
Apolipoprotein A1 |
| 3202 |
Downregulated, p < 0.01 |
71/66 |
8 |
34 |
81/41 |
6(4) |
25 |
Apolipoprotein A1 |
| 3307 |
Upregulated, p < 0.05 |
91/66 |
7 |
36 |
61/58 |
3(1) |
6 |
Haptoglobin |
| 4401 |
Downregulated, p < 0.05 |
68/66 |
6 |
83 |
95/59 |
2(2) |
28 |
Transthyretin chain A |
| 5603 |
Downregulated, p < 0.05 |
71/66 |
6 |
11 |
79/59 |
3(1) |
6 |
Haptoglobin |
| 5604 |
Downregulated, p < 0.01 |
82/66 |
11 |
49 |
84/58 |
2(1) |
4 |
Haptoglobin |
| 6203 |
Downregulated, p < 0.05 |
127/66 |
17 |
65 |
76/58 |
6(3) |
29 |
Apolipoprotein A1 |
| 7102 |
Upregulated, p < 0.01 |
55/66 |
8 |
-- |
64/56 |
2(1) |
13 |
Haptoglobin (alpha2) |
| 7201 |
Downregulated, p < 0.01 |
106/66 |
16 |
66 |
76/59 |
3(2) |
13 |
Apolipoprotein A1 |
| 7503 |
Downregulated, p < 0.05 |
53/66 |
5 |
-- |
64/58 |
2(2) |
7 |
Vitamin D binding protein |
|
8102 |
Upregulated, p < 0.01 |
68/66 |
5 |
20 |
70/57 |
3(1) |
8
|
Haptoglobin (alpha2)
|
| 9101 | Upregulated, p < 0.05 | 49/66 | 3 | -- | 105/55 | 1(1) | 4 | Haptoglobin (alpha2) |
Protein spots displaying significant intensity differences between obese children (OB) and controls (C) prior to the false discovery rate (FDR) analysis. The spot in bold (8102) remained significantly different after FDR analysis. The second column shows the direction of the change observed in OBvs.C. Abbreviations:MS Mass spectrometry; MS-MS Tandem mass spectrometry.