Table 3.
Protein spot displaying intensity differences (p < 0.05) between insulin resistant (IR) and non-IR obese children before FDR analysis
| Spot |
Spot intensity in IR,
|
MS
|
MS
|
MS
|
MS -MS
|
MS-MS
|
MS-MS
|
Identification |
|---|---|---|---|---|---|---|---|---|
| Significance level | Score/Cut-off | Matched fragments | Sequence coverage (%) | Score/Cut-off | Matched fragments | Sequence coverage (%) | ||
| 1703 |
Upregulated, p < 0.05 |
111/66 |
15 |
54 |
100/40 |
6(4) |
26 |
Alpha-1-Antitrypsin |
| 3002 |
Upregulated, p < 0.05 |
48/66 |
4 |
-- |
52/38 |
1(1) |
4 |
Haptoglobin |
| 3101 |
Downregulated, p < 0.05 |
160/66 |
20 |
69 |
116/40 |
11(8) |
43 |
Apolipoprotein A1 |
| 3301 |
Downregulated, p < 0.05 |
85/66 |
7 |
22 |
79/41 |
5(4) |
23 |
Clusterin (Apo J) |
|
4101 |
Downregulated, p < 0.01 |
165/66 |
23 |
73 |
150/49 |
11(5) |
43 |
Apolipoprotein A1
|
| 4103 |
Downregulated, p < 0.05 |
161/66 |
22 |
73 |
171/59 |
11(4) |
43 |
Apolipoprotein A1 |
| 4402 |
Downregulated, p < 0.05 |
49/66 |
5 |
-- |
97/58 |
1(1) |
4 |
Clusterin (Apo J) |
| 4503 |
Downregulated, p < 0.05 |
93/66 |
13 |
33 |
72/59 |
6(2) |
15 |
Haptoglobin |
| 5503 |
Downregulated, p < 0.05 |
56/66 |
6 |
-- |
81/59 |
2(0) |
4 |
Haptoglobin |
| 7101 |
Upregulated, p < 0.05 |
74/66 |
6 |
20 |
99/56 |
2(1) |
7 |
Haptoglobin |
|
8101 |
Upregulated, p < 0.05 |
55/66 |
5 |
-- |
73/56 |
2(1) |
13
|
Haptoglobin
|
| 9101 | Upregulated, p < 0.05 | 49/66 | 3 | -- | 105/55 | 1(1) | 4 | Haptoglobin |
The second column shows the direction of the change observed in IR vs. non-IR. Abbreviations:MS Mass spectrometry, MS-MS Tandem mass spectrometry.
Spots in bold remained significant after FDR analysis.