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. 2014 Jan;5(1-2):31–40. doi: 10.18632/genesandcancer.2

Figure 3. E2F1 promoter regulation by CtBP.

Figure 3

A. Diagram of E2F1-Luc and E2F1-mu-Luc constructs. The E2F1 promoter region from (−)318 to (+)36 was PCR amplified and cloned into the pG5-Luc vector. The two E2F motifs in this promoter region were mutated as indicated at the bottom. B. E2F1-Luc was co-transfected with Myc-E2F7 or Myc-E2F7(DL→AS) mutant at indicated amounts, and Luc activity was determined. Only Luc data was plotted since Myc-E2F7 caused a severe repression of hRL-tk internal control. The expression of wt and mutant E2F7 proteins is shown at the bottom.