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. 2013 May 7;31(8):1511–1522. doi: 10.1002/stem.1426

Figure 1.

Figure 1

NotchIC amplifies STAT3 activity in embryonic stem (ES) cells. (A): Western blot analysis for phospho-Stat3 and total Stat3 in R26NotchIC cells or clonally related parental control cells cultured in LIF plus fetal calf serum. (B): Quantitative polymerase chain reaction analysis for Socs3 in R26NotchIC cells or clonally related parental control cells plated in N2B27 serum-free media overnight and then stimulated with LIF for 2 hours. (C): Normalized luciferase activity in D3 parental cells or RBPJk homozygous null ES cells after transfection with a STAT3-luciferase plasmid and an SV40-renilla plasmid together with a γ-secretase-dependent form of NotchIC (pNotchICΔE) or empty vector plasmid, either in the presence of absence of 4 μM GSI. All data are represented as mean ± SD. *Indicates p < .05 relative to parental or empty vector controls. Abbreviation: GSI, gamma secretase inhibitor; WT, wild type.