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. 2014 Apr 14;86(12):5601–5605. doi: 10.1021/ac500952z

Figure 4.

Figure 4

PHIP of 1-13C-PEP-d2 to HP 1-13C-PLAC-d2. (a) Diagram of molecular hydrogenation of 1-13C-PEP-d2, deuterium enrichment of 95% (SI, section 2.4), with parahydrogen to form HP 1-13C-PLAC-d2. (b) 13C spectrum of PHIP HP 1-13C-PLAC-d2 (52 μmoles in ∼3 mL and ∼12 μmoles of unreacted 1-13C-PEP-d2 corresponding to ∼82% conversion) acquired at 5.75 mT in situ of the automated PHIP polarizer using 7 atm of parahydrogen and ∼92 °C. (c) Signal reference 13C spectrum of sodium 1-13C-acetate (165 mmol in 52 mL of D2O) with polarization P = 4.70 × 10–4% at acquisition. (d) T1 decay of 13C hyperpolarization of HP 1-13C-PLAC-d2.