Figure 3.

Effect of the GPX4c718t SNP in monocytes and EC fatty acid pretreatment on adhesion to HUVECs. Monocytes from volunteers genotyped for GPX4c718t (CC or TT) were isolated and added to HUVECs grown in the presence or absence of Se (+Se and −Se) and challenged with TNF-α and the number of adhering monocytes was assayed. Cells were either pretreated with no fatty acid (A) or pretreated with either linoleic acid (B) or ARA (C) at 10 μM for 48 h. Values are expressed as means ± SEM. Statistical analysis was carried out by four-way ANOVA on the log-transformed data followed by post hoc t-tests (*p < 0.05, **p < 0.01 and ***p < 0.001).