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. Author manuscript; available in PMC: 2015 Apr 18.
Published in final edited form as: ACS Chem Biol. 2014 Feb 10;9(4):957–966. doi: 10.1021/cb400898t

Figure 5. The basic C-terminus is a remote site of allosteric, activating binding by Hep dp16 oligosaccharides.

Figure 5

(A) ProMMP-7 (5.5 µM) with wt sequence or deletion of KRSNSRKK was incubated with increasing concentrations of Hep dp16 at 37 °C for 2 h until each reaction was stopped. (B) The synthetic peptide with the KRSNSRKK sequence from the C-terminus interferes in Hep dp16-induced activation of proMMP-7. (C) Hep dp16 effects on catalytic efficiency (kcat/KM) and proteolytic turnover (kcat) of soluble Knight's peptide were monitored by FRET (25 °C, pH 7.5) are compared between active wt-MMP-7 and MMP-7ΔC.