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. 2014 Jun 5;2:19. doi: 10.1186/2049-2618-2-19

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Copyright © 2014 Wesolowska-Andersen et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Comparison of abundance estimations for bacterial genera with MetaHIT and HMP methods. (A) Relative abundance of the most prevalent bacterial genera with MetaHIT and HMP DNA extraction methods. The horizontal line is plotted at a value of 0.5, corresponding to equal abundance of a given genus detected by both methods. (B) Estimated abundance of the 25 most abundant bacterial genera with established taxonomy mapping shows clear differences for several genera. The most significant differences are observed for Bacteroidetes; for three out of six cases the HMP method resulted in higher numbers of reads mapped to respective species. (*:P < 0.05; **:P < 0.01; ***:P < 0.001).