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. Author manuscript; available in PMC: 2014 Jun 19.
Published in final edited form as: Am J Physiol. 1986 Jun;250(6 0 2):H1060–H1070. doi: 10.1152/ajpheart.1986.250.6.H1060

TABLE 1.

Association of IDMI with plasma proteins

Protein Fraction % Protein Precipitated %IDMI in Fraction
Albumin 56.7 81.9
α- and β-Globulins 12.0 8.8
α-, β-, and γ-Globulins 7.7 1.3
γ-Globulins 23.6 6.4
Supernatant after TCA addition 1.6

Ammonium sulfate was used to precipitate the plasma proteins differentially (Fruton and Simmonds, 1958). Trichloroacetic acid (TCA, 15% final concentration) was used to ensure complete precipitation. Plasma was obtained from heparinized human blood (24°C). IDMI, 2-iododesmethylimipramine.