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. 2014 Apr 16;8(1):211–217. doi: 10.3892/ol.2014.2071

Figure 4.

Figure 4

Effect of angiopoietin-like 4 (ANGPTL4) on human aldolase A (ALDOA) promoter activities. WM-115 and WM-266-4 cells were transfected with human ALDOA promoter-luciferase reporter plasmids. After 24 h, luciferase assays were performed. WM-115 cells: Luciferase activity in control cells, cells stably transfected with empty pcDNA3 vector, cells overexpressing ANGPTL4, cells treated with selective protein kinase C (PKC) inhibitor Go6983 (500 nM, 24 h), and cells overexpressing ANGPTL4 and simultaneously treated with Go6983 (500 nM, 24 h), was analyzed. WM-266-4 cells: Luciferase activity in control cells, cells stably transduced with scramble control shRNA, cells stably expressing ANGPTL4-shRNA, cells treated with selective PKC agonist phorbol 12-myristate 13-acetate (PMA; 500 nM, 24 h), and cells stably expressing ANGPTL4-shRNA and simultaneously treated with PMA (500 nM, 24 h), was analyzed. The luciferase activity was expressed as the fold-change to that of WM-115 control cells (designated as 1). WM-115 cells: αP<0.05, compared with Control and Vector; βP<0.05, compared with ANGPTL4; γP<0.05, compared with PKC inhibitor. WM-266-4 cells: δP<0.05, compared with Control and Scramble; χP<0.05, compared with ANGPTL4-shRNA.