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Effect of DAC and ATRA on the early apoptosis of K562 cells by flow cytometry. K562 cells were treated with the two drugs for 48 h. Apoptosis was assayed by Annexin V staining and fluorescence-activated cell sorting analysis. The early apoptotic cells (sorted only by Annexin V) are found in the bottom right gate. DAC, 5-aza-2′-deoxycytidine; ATRA, all-trans retinoic acid; FITC, fluorescein isothiocyanate.
