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. 2014 Jun 19;10(6):e1004343. doi: 10.1371/journal.pgen.1004343

Figure 5. Ecdysone and 20E efficiently rescue loss of vvl and kni in the PG.

Figure 5

(A) Ecdysone biosynthetic scheme showing steps in the conversion of cholesterol to 20-hydroxyecdysone (20E). Note that ecdysone produced and released from the PG is converted to its active form 20E in peripheral tissues. (B) Percentage of larvae developing to the indicated stage. L1; first instar larvae, L2; second instar larvae, L3; third instar larvae. Resupplying precursors later in the pathway is gradually more efficient in rescuing arrest of larvae with reduced expression of vvl and kni in the PG. In contrast, only precursors downstream of the black box efficiently rescue mld-RNAi larvae, indicating that Mld regulates a gene product(s) involved in the reactions upstream of the 5β-ketodiol. C; cholesterol, 7dC; 7-dehydrocholesterol, KD; 5β-ketodiol, KT; 5β-ketotriol, 2dE; 2-deoxyecdysone, E; ecdysone, 20E; 20-hydroxyecdysone.