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. 2014 Jun 19;10(6):e1004368. doi: 10.1371/journal.pgen.1004368

Figure 8. Translational regulation by the Ier3 3′UTR.

Figure 8

(A) Diagram depicting the similarity of regulatory motifs between the Ier3 and Tnf genes. Shared transcriptional and post-transcriptional elements are highlighted. (B) HEK-Blue mTLR4 cells were transiently co-transfected with different Firefly Luciferase reporter genes together with a Renilla Luciferase expressing plasmid. Firefly Luciferase reporter genes contained either the rabbit β-globin (HBB2) 3′UTR alone, the complete Ier3 3′UTR or the Ier3 3′UTR without the ARE. (C) To determine the relative translation efficiency, Firefly Luciferase (FLuc) activity was normalized to Renilla Luciferase (RLuc) activity and to the Firefly Luciferase reporter mRNA level as determined by Northern blot analysis. Bars represent mean values ± SEM (n = 4).