Figure 9. Effect of Ier3 knockout on TNF production and survival of LPS-stimulated BMDM.

(A) TNF secretion by wild type and Ier3 knockout BMDM was measured using a bead-based flow cytometry assay. Mean values ± SEM were determined from four experiments using BMDM of two different pairs of wild type and knockout mice. (B) Cell death was measured after propidium iodide and Annexin V staining by flow cytometry. Depicted is the mean percentage of double positive cells (± SEM) based on five experiments using BMDM from three different pairs of wild type/knockout mice.