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. 2014 Jun 19;10(6):e1004415. doi: 10.1371/journal.pgen.1004415

Figure 1. Swd2.2 antagonizes the association of Condensin with chromatin.

Figure 1

A. Serial dilutions of the indicated strains were plated on rich media at the indicated temperatures. B. Chromosome segregation in anaphase was visualized after tubulin staining in cells of the indicated genotypes grown for one generation at 34°C. C. Anaphases were scored as defective when chromatin was detected lagging between the two main DNA masses. For each genotype, a minimum of 6 independent experiments was performed in which a minimum of 100 anaphase cells was scored. ***p<0,001 Wilcoxon - Mann Whitney. D and E. Same as A and C, except that in E, 3 independent experiments were performed. F. The indicated strains were grown at 34°C for 3 hours and ChIP-qPCR was performed to analyze the amount of Cut14-GFP cross-linked to chromatin (mean ± standard deviation from 6 biological replicates). See text for details for the statistical analysis of the experiments.