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. 2014 Jun 19;10(6):e1004415. doi: 10.1371/journal.pgen.1004415

Figure 2. Lack of Swd2.2 reduces the association of the RNA Pol III transcription machinery with chromatin.

Figure 2

A. Asynchronous populations of the indicated strains were grown at 30°C and ChIP-qPCR was performed to analyze the amount of Swd2.2-3flag cross-linked to chromatin (mean ± standard deviation from 4 biological replicates). BC. ChIP qPCR analysis of the indicated strains grown at 30°C. Mean ± standard deviation from 5 biological replicates. *<0,05; **<0,01; Wilcoxon - Mann Whitney. D. Western blot analysis of total protein extracts of the indicated strains. Tubulin (TAT1 antibody) is used as a loading control. E. qRT-PCR analysis of sfc6 expression in swd2.2+ and swd2.2Δ cells (mean ± standard deviation from 3 biological replicates) F. Serial dilutions of the indicated strains were plated on rich media at the indicated temperatures.