Skip to main content
. 2014 Jun 19;10(6):e1004433. doi: 10.1371/journal.pgen.1004433

Figure 1. Identification and complementation of PBP1A pathway mutants using a chemical genetic screen.

Figure 1

(A) For the screen, an ordered V. cholerae transposon library was pintooled onto LB agar +/−5 mM D-Met and scored for growth deficiency in the presence of D-Met. (B) Growth of V. cholerae PBP1A pathway mutants containing either an empty vector (pHL) or a plasmid enabling inducible expression of the deleted gene (pHLorf) on plates containing inducer and 5 mM D-Met confirms that their growth deficiencies on D-Met are due to deletion of the indicated genes.