Figure 3. A csiV mutant phenocopies the growth deficiencies of mrcA and lpoA mutants.

The minimum inhibitory concentration (MIC) of (A) deoxycholate, (B) cefsulodin and (C) DAA are shown for wt V. cholerae and PBP1A pathway mutants. MIC data reflect values obtained with two biological replicates done in technical quadruplicates for each strain. Absence of error reflects that the same values were obtained for all experiments (D) Morphological changes induced by DAA. The indicated V. cholerae strains were grown in the absence of DAA until OD₆₀₀∼0.3 and then applied to 0.8% agarose pads containing 4 mM D-Met. Scale bar = 5 µm.