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. 2014 Jun 19;10(6):e1004433. doi: 10.1371/journal.pgen.1004433

Figure 4. Muropeptide analysis of the csiV, mrcA and lpoA mutants.

Figure 4

Cell wall content was estimated by colorimetric quantification of DAP in PG extracted from (A) exponential phase or (B) stationary phase cultures. (C–F) UPLC analysis of the degree of crosslinking (C,D) and average chain length (E, F) in cell wall extracted from exponential phase (C,E) and stationary phase (D,F) cultures. Data are averages of two (exponential phase) or three (stationary phase) biological replicates, error bars indicate standard deviation. ** = p<0.01 (t-test). S481T is a PBP1A transpeptidase mutant expressed from its native chromosomal locus, where the S residue at position 481 (in the active site) is replaced by a T.