Figure 6. Photomicrographs of immunostaining showing expression of the membrane markers ZO-1 A), Na⁺K⁺/ATPase (B), connexin 43 (C), and N-cadherin (D) immunostaining of a confluent layer of corneal endothelial cells (CECs) isolated and expanded in DMEM + F12 medium supplemented with 10% heat-treated platelet releasate (HPR).

(Cell nuclear markers were stained with DAPI. Proteins were clearly localized at plasma membranes of cells with a hexagonal shape (magnification 200x).