Table 1. Comparison of different medium conditions for in(CECs).

Condition	1	2	3	4	5	6	7
Medium Base	DMEM + F12	DMEM + F12	DMEM + F12	DMEM + F12	DMEM + F12	DMEM + F12	DMEM + F12
FBS	5%	5%	−	−	−	−	−
Human platelet lysate	−	−	5% PR	5% HPR	5% HPR	5% PR	5% HPR
7.5% Sodium Bicarbonate	+	+	+	+	+	+	+
Heparin	−	+	+	+	−	+	+
0.5% DMSO	+	+	−	−	−	+	+
2 ng/ml rHuEGF	+	+	−	−	−	−	−
5 µg/ml Insulin; +5 µg/mlTransferrin; +5 ng/ml Selenium	+	+	−	−	−	−	−
1 nM Cholera Toxin	+	+	−	−	−	−	−
1% Amp B; +1% Pen- Strep	+	+	+	+	+	+	+

DMEM, Dulbecco’s modified Eagle medium; PR, platelet releasate; HPR, heat-treated platelet lysate; rHuEGF, recombinant human epithelial growth factor.