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. 2014 Jun 19;9(6):e100250. doi: 10.1371/journal.pone.0100250

Figure 3. An example of parental dSNP and tri-hybrid ASGE validation.

Figure 3

The represented transcript is ENSORLT00000013099 (ensembl Transcript ID). One dSNP per parental genome is represented. (A) RNA-seq and Sanger obtained sequence alignments between the parental strains (SOK, HNI-II and OR) and the Sanger obtained sequences for both trpA and trpB triploids. (B) Aligned chromatograms of the three parental strains and trpA and trpB. In both triploids the allele expression pattern determined by Sanger is consistent with the one obtained by RNA-Seq for this transcript. In this example SOK and HNI-II dSNPs are observed in the triploid, but the OR dSNP is not observed.