Table 4. Oligonucleotides used for plasmid content analysis.
Primer name | Primer sequence | Predicted fragment size (bp) | Plasmid nucleotide coordinates |
lp36A-Forward | 5′-GGG AAA TGC TAT AAG CCT CAA GCC CAG CC-3′ | 2480 | 26244–28724 |
lp36A-Reverse | 5′-GCT TAG CCT GCA CCA AGC TAT TGC TAA GG-3′ | ||
lp36B-Forward | 5′-GCATCTCCTATAAGTATAC-3′ | 184 | 27069–27253 |
lp36B-Reverse | 5′-TCCCTGGTAAAGTAATTCATTTATCC-3′ | ||
BBK19-Forward | 5′-CTA TTG TTT GTC CAA GTC TTA ATC-3′ | 1196 | 12269–13465 |
BBK19-Reverse | 5′-ATT GTA CGA AGT TGA ATT TTA GCA TC-3′ | ||
OspA-Forward | 5′-GGG AAT AGG TCT AAT ATT AGC C-3′ | 770 | 9410–10160 |
OspA-Reverse | 5′-TTT CAA CTG CTG ACC CCT C-3′ |