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. 2014 Jun 19;9(6):e99950. doi: 10.1371/journal.pone.0099950

Figure 2. Isoflurane transiently increases CD73 activity without changing CD73 synthesis in cultured endothelial cells.

Figure 2

A. Human umbilical vein endothelial (EA.hy926) cells treated with 2.5% isoflurane showed a significant but transient induction of CD73 activity. CD73 activity peaked at 3 hr and then decreased to near baseline at 6–16 hr after isoflurane treatment (N = 6–8). B. Isoflurane treatment for 3 hr caused dose-dependent increase in CD73 activity in EA.hy926 cells compared to carrier gas-treated cells (N = 4–5). Data are presented as means ± SEM. *P<0.05 vs. CD73 activity measured at baseline (A) or in cells treated with 0% isoflurane (B). C and D. Representative images for CD73 mRNA (RT-PCR) and protein (immunoblotting) expression in EA.hy926 cells. EA.hy926 cells were treated with carrier gas or with 2.5% isoflurane for 6 hr (C) or for 16 hr (D). Isoflurane treatment did not increase CD73 mRNA or protein expression in EA.hy926 cells. Representative of 3–4 experiments.