Figure 5.

Phosphorylation of AKT controls the proliferation of gemcitabine-resistant breast cancer cells.

Notes: Expression of p-AKT, p-MEK, p-p70S6K and p-mTOR was detected by Western blot in 231/Gem cultured with different inhibitory treatments for 2 hours. The activation of p-p70S6K indicates the activity of p-mTOR.

Abbreviations: 231/Gem, chemoresistant human breast cancer cell line MDA-MB-231/gemcitabine; AKT, protein kinase B; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; MEK, mitogen-activated protein kinase kinase; mTOR, mammalian target of rapamycin; p, phosphorylated.