Table 1. Bacterial strains and plasmids used in this study.
| Strain/plasmid | Relevant description | Source |
|---|---|---|
| Strains | ||
| C. jejuni 81–176 | Used as wild type strain of C. jejuni | Dr Qijing Zhang |
| E. coli DH5α | E. coli strain used for cloning | Invitrogen |
| ∆ppx1 | C. jejuni 81–176 derivative with deletion in ppx1/gppa gene; ppx1/gppa::Kan | This study |
| ∆ppx2 | C. jejuni 81–176 derivative with deletion in ppx2/gppa gene; ppx2/gppa::Kan | This study |
| dkppx | C. jejuni 81–176 derivative with deletion of ppx1/gppa and ppx2/gppa; ppx1/gppa::Kan, ppx2/gppa::Cm | This study |
| Cppx1 | ∆ppx1 with pRY111- containing ppx1coding region and the upstream promoter sequence for complementation; Cm | This study |
| Cppx2 | ∆ppx2 with pRY111- containing ppx2 coding region and the upstream promoter sequence for complementation; Cm | This study |
| Plasmids | ||
| pRY111 | E. coli-Campylobacter shuttle vector for complementation | Yao et al.50 |
| pRK2013 | Helper plasmid for conjugation, Kan | Akiba et al.58 |
| pZErO-1 | Cloning vector for making suicide vector; Zeo | Invitrogen |
| pUC4K | Source for kanamycin gene, Kan | Amersham |
| pZErO-1-ppx1 | pZErO-1 containing ppx1/gppa region plus 1 kb upstream and downstream sequences from C. jejuni 81–176; Zeo | This study |
| ppx1-suicide vector | pZErO-1-ppx1 with ppx1/gppa replaced by kanamycin resistance gene from pUC4K; Zeo, Kan | This study |
| pZErO-1-ppx2 | pZErO-1 containing ppx2/gppa region plus 1 kb upstream and downstream sequences from C. jejuni 81–176; Zeo | This study |
| ppx2-suicide vector | pZErO-1-ppx2 with ppx2/gppa replaced by kanamycin resistance gene from pUC4K; Zeo, Kan | This study |
| ppx2-suicide vector-Cm | ppx2-suicide vector with kanamycin resistance marker replaced by chloramphenicol resistance gene from pRY111; Zeo, Cm | This study |
| pRY111-ppx1 | pRY111 containing ppx1 coding and upstream sequence for complementation, Cm | This study |
| pRY111-ppx2 | pRY111 containing ppx2 coding and upstream sequence for complementation, Cm | This study |
Kan, kanamycin; Zeo, zeocin; Cm, chloramphenicol