Table 1. Bacterial strains and plasmids used in this study.
Strain/plasmid | Relevant description | Source |
---|---|---|
Strains | ||
C. jejuni 81–176 | Used as wild type strain of C. jejuni | Dr Qijing Zhang |
E. coli DH5α | E. coli strain used for cloning | Invitrogen |
∆ppx1 | C. jejuni 81–176 derivative with deletion in ppx1/gppa gene; ppx1/gppa::Kan | This study |
∆ppx2 | C. jejuni 81–176 derivative with deletion in ppx2/gppa gene; ppx2/gppa::Kan | This study |
dkppx | C. jejuni 81–176 derivative with deletion of ppx1/gppa and ppx2/gppa; ppx1/gppa::Kan, ppx2/gppa::Cm | This study |
Cppx1 | ∆ppx1 with pRY111- containing ppx1coding region and the upstream promoter sequence for complementation; Cm | This study |
Cppx2 | ∆ppx2 with pRY111- containing ppx2 coding region and the upstream promoter sequence for complementation; Cm | This study |
Plasmids | ||
pRY111 | E. coli-Campylobacter shuttle vector for complementation | Yao et al.50 |
pRK2013 | Helper plasmid for conjugation, Kan | Akiba et al.58 |
pZErO-1 | Cloning vector for making suicide vector; Zeo | Invitrogen |
pUC4K | Source for kanamycin gene, Kan | Amersham |
pZErO-1-ppx1 | pZErO-1 containing ppx1/gppa region plus 1 kb upstream and downstream sequences from C. jejuni 81–176; Zeo | This study |
ppx1-suicide vector | pZErO-1-ppx1 with ppx1/gppa replaced by kanamycin resistance gene from pUC4K; Zeo, Kan | This study |
pZErO-1-ppx2 | pZErO-1 containing ppx2/gppa region plus 1 kb upstream and downstream sequences from C. jejuni 81–176; Zeo | This study |
ppx2-suicide vector | pZErO-1-ppx2 with ppx2/gppa replaced by kanamycin resistance gene from pUC4K; Zeo, Kan | This study |
ppx2-suicide vector-Cm | ppx2-suicide vector with kanamycin resistance marker replaced by chloramphenicol resistance gene from pRY111; Zeo, Cm | This study |
pRY111-ppx1 | pRY111 containing ppx1 coding and upstream sequence for complementation, Cm | This study |
pRY111-ppx2 | pRY111 containing ppx2 coding and upstream sequence for complementation, Cm | This study |
Kan, kanamycin; Zeo, zeocin; Cm, chloramphenicol