Figure 4. LysPG loss results in decreased survival of L. monocytogenes in the presence of bile. Colony forming units/mL remain the same for L. monocytogenes EGD-e (red) and L. monocytogenes EGD-e ΔlysPGS::lysPGS (black) after incubation in 24% bile over the course of 4 h, while the survival rate of L. monocytogenes EGD-e ΔlysPGS (blue) decreases as does that of positive control, L. monocytogenes EGD-e Δbsh (green). Data represent averages of three independent experiments and the corresponding standard errors.