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. 2014 Jun 13;9:2919–2932. doi: 10.2147/IJN.S59799

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© 2014 Zhang et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Subcellular localization of different FITC-labeled NPs evaluated by confocal laser scanning microscopy after incubation with (A) Ost/FITC-NSC-NPs, 30 minutes; (B) Ost/FITC-LDL-NSC-NPs, 30 minutes; (C) Ost/FITC-NSC-NPs, 1 hour; (D) Ost/FITC-LDL-NSC-NPs, 1 hour; (E) Ost/FITC-NSC-NPs, 3 hours; and (F) Ost/FITC-LDL-NSC-NPs, 3 hours.

Notes: FITC-labeled NPs, green (a); cell nuclei stained with Hoechst 33258, blue (b); overlay of (a) and (b) (c).

Abbreviations: FITC, fluorescein isothiocyanate; LDL, low-density lipoprotein; NPs, nanoparticles; NSC, N-succinyl-chitosan; Ost, osthole.