Figure 5. Influence of androgens on imprinted genes expression. Expression of the indicated imprinted genes was measured relative to TBP mRNA by qRT-PCR in LNCaP cells treated for 3, 8 or 24 h with 10 nM synthetic androgen R1881 (black boxes) or with solvent only (gray circles). Treatments were performed in biological duplicates and qRT-PCR was performed in duplicate for each sample, whereby less than 10% variation between duplicates was accepted. The data points with error bars represent mean values from the biological duplicates normalized to the expression of the untreated control at 3 h that was set to 100%. Standard deviation is presented as error bars. *Significant changes (P < 0.05) as calculated by T-Test.