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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Stem Cells. 2014 Jul;32(7):1746–1758. doi: 10.1002/stem.1716

Figure 5. Alternative inhibition of CD36, by MTN, results in decreased CSC self-renewal.

Figure 5

MTN treatment (red) of CSCs reduced self-renewal capacity (A) and stem cell frequencies (B) compared to DMSO treated controls (black). Effects of MTN treatment were specific to CSCs vs non-CSCs. Immunoblotting analysis (C) CSCs from patient-derived GBM xenografts (T3832, GBM10) treated with MTN for 18 hours indicates that CSC self-renewal pathways (Sox2, p-Akt, p-Stat 3) were decreased. Actin was used as a loading control. MTN treatment (red) specifically induced apoptosis as compared to the DMSO control (black), measured by Caspase 3/7 activity (D) in CSCs after 3 days of treatment. All limiting dilution analyses were performed in triplicate and p-value indicated on each graph. Caspase measurements are represented as a mean +/− standard deviation and conducted in duplicate.