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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Stem Cells. 2014 Jul;32(7):1746–1758. doi: 10.1002/stem.1716

Figure 6. Oxidized lipid uptake is elevated in CSCs.

Figure 6

Immunofluorescence images (A) of human GBM tissue (CCF860) demonstrates detectable oxidized lipid species (ox lipids, red) adjacent to CD36 positive cells (green) in perivascular regions (CD31, white). Cell growth analysis (B) of CSCs and non-CSCs derived from patient-derived GBM xenografts (T3832, GBM10) demonstrates that CSC growth is increased by administration of 50 µg/ml for 16 hours in oxidized low density lipoprotein (oxLDL, red) conditions as compared to low density lipoprotein (LDL, grey) and buffer (black) conditions. CD36 siRNA did not reduce the growth in the oxLDL as compared to the LDL condition in CSCs, which was observed in control siRNA treatment (C). Immunofluorescence staining performed in triplicate and observed in tissue from an additional GBM patient. Nuclei in micrographs were counterstained with dapi (blue), yellow arrow indicates inset area, and scale bar represents 20 µm. Growth assays performed in at least duplicate and represent mean +/− standard deviation. P-values are indicated on graphs where significant: * p<0.05, ** p<0.01, *** p<0.001.