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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Angiogenesis. 2014 Feb 23;17(3):675–683. doi: 10.1007/s10456-014-9423-8

Figure 6. Quantitative RT-PCR analysis of ANGPTL2 and MT1-MMP gene expression from 3D ECFC vascular structures.

Figure 6

3D vascular structures formed from siRNA treated ECFCs were lysed with RNA lysis buffer at 48 hours. Real time PCR was used to determine relative expression levels of ANGPTL2 and MT1-MMP in 3D in response to ANGPTL2 KD. Both transcripts were found to be decreased in ANGPTL2 silenced ECFCs in 3D. Data are normalized to the housekeeping gene ATP5B. RQ = relative quantity; n = 3. Statistical analysis: Mann-Whitney test.