Skip to main content
. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Metab Brain Dis. 2013 Dec 19;29(4):1027–1039. doi: 10.1007/s11011-013-9469-0

Table 1.

Primary antibodies

Primary Antibody Company Species raised in Catalogue Number Section thickness (μm) Heat-Induced Epitope Retrieval Antibody dilution
Proliferating Cell Nuclear Antigen Santa Cruz Biotechnology, California, USA Mouse monoclonal sc-56 48 10mM sodium citrate buffer pH 8.5 at 60°C overnight 1:750
7 10mM Tris 1mM EDTA pH 9.0 at 95°C for 30 minutes 1:500
Ionized calcium binding adaptor molecule 1 Wako Pure Chemicals, Osaka, Japan Rabbit polyclonal 019-19741 48 None required 1:1000
7 10mM Tris 1mM EDTA pH 9.0 at 95°C for 30 minutes 1:1000
Ki-67 (Clone MIB-1) Dako, Denmark Mouse monoclonal M7240 7 10mM Tris 1mM EDTA pH 9.0 at 110°C for 30 minutes 1:500
Beta III Tubulin Abcam, Cambridge, USA Rabbit polyclonal ab18207 7 10mM Tris 1mM EDTA pH 9.0 at 110°C for 30 minutes 1:200
GFAP Dako, Denmark Rabbit polyclonal Z0334 7 10mM Tris 1mM EDTA pH 9.0 at 110°C for 30 minutes 1:500
NeuN Millipore, Billerica, USA Rabbit polyclonal ABN78 7 10mM Tris 1mM EDTA pH 9.0 at 110°C for 30 minutes 1:200