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. 2013 Dec 6;197(2):471–483. doi: 10.1534/genetics.113.159210

Figure 6.

Figure 6

Notch activity is reduced after wasp infection. Anti-β-galactosidase antibody (red)-stained lobes from Su(H)-lacZ animals costained with anti-pro-PO (green) to detect coincidence of high Notch activity in crystal cells (stars). Cells expressing Su(H)-lacZ only make up 25.3% of all labeled cells; cells positive for pro-PO only constitute 37.3% of all labeled cells. The remainder labeled cell population (37.3%) are double positive (n = 406 cells from 20 lobes). Control animals did not experience wasp attack (A–C′), or animals were exposed to wasps (D–F) before dissection. Upon infection, Notch activity decreases (D–G). Standard deviation is shown in G. (H and I) Lamellocyte differentiation in Su(H)-lacZ lobes is normal. Anterior lobes are from uninfected Su(H)-lacZ (H) or infected (I) animal. Lamellocytes are labeled with phalloidin tagged with Alexa Fluor-647 (white), which also labels the dorsal vessel.