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Overexpression studies demonstrate that Eftud2 and Atp6v1c1 regulate IL-6 expression. Plasmids overexpressing the indicated genes were cotransfected along with plasmids expressing IL-6-luciferase and SV40-rluc into RAW264.7 cells, the cells were stimulated with the indicated quantities of LPS for 6 hr, and IL-6-luc activity (normalized to SV40-rluc) was monitored.
