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. 2013 Dec 20;197(2):485–496. doi: 10.1534/genetics.113.160499

Figure 5.

Figure 5

Eftud2 regulates the innate immune response in mouse macrophages. (A–C) Cells were treated with either Eftud2 siRNA or control (CT) siRNA; were exposed to 20 ng/ml LPS for 6 hr; and IL-6 protein (A), IL-6 mRNA (B), or TNFα protein (C) was monitored. (D) Cells were transiently transfected with plasmids expressing IL-6-luciferase, SV40-rluc, and either Eftud2 or CAT; were exposed to 50 ng/ml LPS for 6 hr where indicated; and IL-6-luc production (normalized relative to SV40-rluc) was monitored. (E and F) Cell lines stably overexpressing Eftud2, CAT, or the parent RAW264.7 cell line were stimulated with 20 ng/ml LPS for 6 hr and cytokine production was monitored.