Figure 4.

Rescue of slpr mutant viability or dorsal closure demonstrates kinase specificity. (A) Floating bar plot showing the degree of rescue provided by expression of the indicated transgenes (x-axis), as a ratio of slpr^BS06 mutant to sibling FM7c male flies (y-axis). Bars span minimum to maximum values and horizontal lines indicate the mean ratio for three to six independent trials except Slpr^AAA and S^AAAT^Ct, which were each two trials, testing a minimum of two different transgenic insertions per genotype. In the absence of a UAS construct (no Tg), the eclosion ratio is ∼0.05. The total number (N) of males counted is shown below each bar. Expression of HA-tagged Slpr^WT provides a significant degree of rescue (P < 0.001) using one-way ANOVA with Bonferroni’s multiple comparisons test vs. the control. (B) Bar graph of the phenotype of gt slpr mutant cuticles recovered among progeny of the indicated cross. In the absence of transgene expression, a majority of severe (dorsal and anterior head open) and some moderate (dorsal hole but head in) dorsal open (DO) cuticles are observed. Rescue of dorsal closure by transgene expression (x-axis) decreases the percentages of severe and moderate cuticle phenotypes while increasing the proportions of cuticles with mild (small holes, scabs, head defects) or no defects (∼WT, resembling wild type). The total number (N) of cuticles counted for each genotype is shown above the bars.