Figure 7.

Tak1-dependent antibacterial defense in the absence or presence of ectopic chimera protein expression. (A) Survival curves of Tak1² mutant males after infection with E. coli, without or with expression of indicated transgenes under the control of da-Gal4. Mutant males are susceptible to infection (red) and expression of the transgenic proteins did not significantly rescue the susceptibility. The total number (N) of adult flies tested is shown. (B) Survival curves of females homozygous for Tak1² or heterozygous mutant plus expression of chimeric proteins with the ubiquitous da-Gal4 driver and infected with E. coli. In the absence of transgene expression, homozygous Tak1² females are significantly more susceptible to infection (red) than the heterozygous females (gray), which are not. Expression of dominant-negative Tak1^K46R (light blue) or S^AAAT^Ct (purple) transgenes renders the heterozygous Tak1² females modestly, but significantly, more sensitive than without exogenous protein. The total number (N) of adult flies tested is shown. ***P < 0.0001 according to the log-rank (Mantel–Cox) test.