Figure 8.

The C-terminal region of Tak1 is sufficient to inhibit induction of Rel target gene, Diptericin, in adult females challenged with E. coli. (A) Quantitative real-time PCR results of relative Diptericin (Dpt) antimicrobial gene expression in females expressing the indicated transgenes relative to the Yp1-Gal4 driver-alone control (no Tg) in the absence and presence of bacterial challenge. Values were normalized against RpL32 expression to control for variation in input cDNA and shown as the means ± SEM for three to four independent biological replicates. Statistical comparisons were first performed on each pair (control vs. +Ec) using one-way ANOVA with Bonferroni’s multiple comparisons test. Asterisks indicate significant differences (****P < 0.001) in Dpt induction upon challenge. One-way ANOVA with Bonferroni’s post-test was also used to compare only the values of E. coli challenged groups vs. the control (no Tg +Ec) indicating significant depression of Dpt induction (^##P < 0.01, ^#P < 0.05). (B) Bar graph displaying mean Dpt expression ± SEM values taken from graph in A to compare relative Dpt expression levels in the indicated groups under basal (unchallenged) conditions only. ANOVA analysis comparing all groups to the no Tg control highlights significant induction by Tak1^WT only (****P < 0.001).