Table 2. C-terminal hydrophobic/basic residue pair is required for ciliary localization of a subset of GPCRs.
Fusion proteinb | Neuron | % localized to:a | |||||
---|---|---|---|---|---|---|---|
Cilium | Dendrite, cell body | Cilium, dendrite, cell body, axons | Cell body | No expression | P-valuec | ||
STR-1 | AWB | 100 | 0 | 0 | 0 | 0 | |
STR-1mut | AWB | 0 | 0 | 97 | 0 | 3 | <0.001 |
STR-44 | AWB | 100 | 0 | 0 | 0 | 0 | |
STR-44mut | AWB | 0 | 0 | 100 | 0 | 0 | <0.001 |
STR-163 | AWB | 100 | 0 | 0 | 0 | 0 | |
STR-163mut | AWB | 100 | 0 | 0 | 0 | 0 | |
STR-163 | ASK | 70 | 0 | 0 | 9 | 21 | |
STR-163mut | ASK | 52 | 0 | 0 | 15 | 33 | |
SRSX-3 | AWB | 100 | 0 | 0 | 0 | 0 | |
SRSX-3mut | AWB | 0 | 100d | 0 | 0 | 0 | <0.001 |
SRU-38 | ASK | 81 | 0 | 0 | 3 | 16 | |
SRU-38mut | ASK | 0 | 0 | 0 | 90 | 10 | <0.001 |
SRD-23 | AWB | 100 | 0 | 0 | 0 | 0 | |
SRD-23mut | AWB | 100 | 0 | 0 | 0 | 0 |
Adult animals grown at 20° were examined; n > 30 animals each.
Fusion protein expression was enriched in the indicated subcellular locations, although weaker expression was occasionally detected elsewhere in the cell.
See Figure 2A for sequences mutated in the corresponding receptor proteins.
Differences among proportions in different categories were compared with respective wild-type values in the relevant neuron type for statistical significance. P-values were determined using a χ2 test of independence. Only differences at P < 0.05 or lower are indicated.
The fusion protein was localized to the membrane.