Table 2. C-terminal hydrophobic/basic residue pair is required for ciliary localization of a subset of GPCRs.

Fusion proteinb	Neuron	% localized to:a
		Cilium	Dendrite, cell body	Cilium, dendrite, cell body, axons	Cell body	No expression	P-valuec
STR-1	AWB	100	0	0	0	0
STR-1mut	AWB	0	0	97	0	3	<0.001
STR-44	AWB	100	0	0	0	0
STR-44mut	AWB	0	0	100	0	0	<0.001
STR-163	AWB	100	0	0	0	0
STR-163mut	AWB	100	0	0	0	0
STR-163	ASK	70	0	0	9	21
STR-163mut	ASK	52	0	0	15	33
SRSX-3	AWB	100	0	0	0	0
SRSX-3mut	AWB	0	100d	0	0	0	<0.001
SRU-38	ASK	81	0	0	3	16
SRU-38mut	ASK	0	0	0	90	10	<0.001
SRD-23	AWB	100	0	0	0	0
SRD-23mut	AWB	100	0	0	0	0

Adult animals grown at 20° were examined; n > 30 animals each.

^aFusion protein expression was enriched in the indicated subcellular locations, although weaker expression was occasionally detected elsewhere in the cell.

^bSee Figure 2A for sequences mutated in the corresponding receptor proteins.

^cDifferences among proportions in different categories were compared with respective wild-type values in the relevant neuron type for statistical significance. P-values were determined using a χ² test of independence. Only differences at P < 0.05 or lower are indicated.

^dThe fusion protein was localized to the membrane.