Table 3. Vesicular trafficking and chaperone/adaptor proteins required for localization of GPCR::GFP fusion proteins to AWB and ASK cilia.
Strainb | Fusion protein | Neuron | % localized to:a | |||||
---|---|---|---|---|---|---|---|---|
Cilium | Cilium, PCMC/distal dendrite | Cilium, dendrite, cell body | Cell body | No expression | P-valuec | |||
Wild type | STR-44 | AWB | 100 | 0 | 0 | 0 | 0 | |
STR-163 | AWB | 100 | 0 | 0 | 0 | 0 | ||
STR-163 | ASK | 67 | 0 | 0 | 8 | 25 | ||
SRBC-64 | ASK | 0 | 100 | 0 | 0 | 0 | ||
Vesicular transport | ||||||||
bbs-8(nx77) | STR-44 | AWB | 95 | 0 | 0 | 0 | 5 | |
STR-163 | AWB | 100 | 0 | 0 | 0 | 0 | ||
STR-163 | ASK | 67 | 0 | 0 | 24 | 9 | ||
bbs-1(ok1111) | STR-44 | AWB | 100 | 0 | 0 | 0 | 0 | |
STR-163 | AWB | 100 | 0 | 0 | 0 | 0 | ||
STR-163 | ASK | 48 | 0 | 0 | 19 | 33 | ||
rab-8(tm2526) | STR-44 | AWB | 100 | 0 | 0 | 0 | 0 | |
STR-163 | AWB | 100 | 0 | 0 | 0 | 0 | ||
STR-163 | ASK | 67 | 0 | 0 | 9 | 24 | ||
arl-3(tm1703) | STR-44 | AWB | 96 | 0 | 0 | 0 | 4 | |
STR-163 | AWB | 100 | 0 | 0 | 0 | 0 | ||
STR-163 | ASK | 64 | 0 | 0 | 4 | 32 | ||
arl-13(tm2322) | STR-44 | AWB | 100 | 0 | 0 | 0 | 0 | |
STR-163 | AWB | 100 | 0 | 0 | 0 | 0 | ||
STR-163d | ASK | 0 | 90 | 0 | 0 | 10 | <0.001 | |
SRBC-64 | ASK | 0 | 100 | 0 | 0 | 0 | ||
arl-13(gk513) | STR-44 | AWB | 100 | 0 | 0 | 0 | 0 | |
STR-163 | AWB | 100 | 0 | 0 | 0 | 0 | ||
STR-163 | ASK | 0 | 89 | 0 | 0 | 11 | <0.001 | |
SRBC-64 | ASK | 0 | 100 | 0 | 0 | 0 | ||
Chaperone/adaptor | ||||||||
odr-4(n2144) | STR-44 | AWB | 0 | 0 | 0 | 76 | 24 | <0.001 |
STR-163 | AWB | 100 | 0 | 0 | 0 | 0 | ||
STR-163 | ASK | 80 | 0 | 0 | 20 | 0 | ||
SRBC-64 | ASK | 0 | 100 | 0 | 0 | 0 | ||
unc-101(m1) | STR-44 | AWB | 0 | 0 | 0 | 65 | 35 | <0.001 |
STR-163d | AWB | 100 | 0 | 0 | 0 | 0 | ||
STR-163 | ASK | 0 | 0 | 0 | 12 | 88 | <0.001 | |
SRBC-64 | ASK | 0 | 0 | 57 | 0 | 43 | <0.001 | |
daf-25(m98) | STR-44 | AWB | 0 | 0 | 97 | 3 | 0 | <0.001 |
STR-163 | AWB | 47e | 0 | 6 | 0 | 47 | <0.001 | |
STR-163 | ASK | 0 | 0 | 35 | 30 | 35 | <0.001 | |
SRBC-64 | ASK | 0 | 0 | 78 | 0 | 22 | <0.001 | |
daf-25(m362) | STR-44 | AWB | 0 | 0 | 100 | 0 | 0 | <0.001 |
Adult animals grown at 20° were examined. n ≥ 20 animals. Expression from the same array was examined in wild-type and mutant strains.
Localization patterns were examined in animals exhibiting relatively wild-type ciliary morphology with the exception of unc-101 mutants. AWB and ASK cilia were either truncated or lacked a cilium in AWB in the majority of animals in unc-101 mutants (Dwyer et al. 2001; Kaplan et al. 2010).
Mutant strains were examined together with wild type in each experiment.
Differences among proportions in different categories were compared with respective wild-type values in the relevant neuron type for statistical significance. P-values were determined using a χ2 test of independence. Only differences at P < 0.05 or lower are indicated.
Cilia in these strains were visualized via expression of str-1p::mCherry (AWB) or srbc-66p::mCherry (ASK).
Expression was weak.