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. 2014 Mar 18;197(2):667–684. doi: 10.1534/genetics.114.161349

Table 3. Vesicular trafficking and chaperone/adaptor proteins required for localization of GPCR::GFP fusion proteins to AWB and ASK cilia.

Strainb Fusion protein Neuron % localized to:a
Cilium Cilium, PCMC/distal dendrite Cilium, dendrite, cell body Cell body No expression P-valuec
Wild type STR-44 AWB 100 0 0 0 0
STR-163 AWB 100 0 0 0 0
STR-163 ASK 67 0 0 8 25
SRBC-64 ASK 0 100 0 0 0
Vesicular transport
 bbs-8(nx77) STR-44 AWB 95 0 0 0 5
  STR-163 AWB 100 0 0 0 0
  STR-163 ASK 67 0 0 24 9
 bbs-1(ok1111) STR-44 AWB 100 0 0 0 0
  STR-163 AWB 100 0 0 0 0
  STR-163 ASK 48 0 0 19 33
 rab-8(tm2526) STR-44 AWB 100 0 0 0 0
  STR-163 AWB 100 0 0 0 0
  STR-163 ASK 67 0 0 9 24
 arl-3(tm1703) STR-44 AWB 96 0 0 0 4
  STR-163 AWB 100 0 0 0 0
  STR-163 ASK 64 0 0 4 32
 arl-13(tm2322) STR-44 AWB 100 0 0 0 0
  STR-163 AWB 100 0 0 0 0
  STR-163d ASK 0 90 0 0 10 <0.001
  SRBC-64 ASK 0 100 0 0 0
 arl-13(gk513) STR-44 AWB 100 0 0 0 0
  STR-163 AWB 100 0 0 0 0
  STR-163 ASK 0 89 0 0 11 <0.001
  SRBC-64 ASK 0 100 0 0 0
Chaperone/adaptor
 odr-4(n2144) STR-44 AWB 0 0 0 76 24 <0.001
  STR-163 AWB 100 0 0 0 0
  STR-163 ASK 80 0 0 20 0
  SRBC-64 ASK 0 100 0 0 0
 unc-101(m1) STR-44 AWB 0 0 0 65 35 <0.001
  STR-163d AWB 100 0 0 0 0
  STR-163 ASK 0 0 0 12 88 <0.001
  SRBC-64 ASK 0 0 57 0 43 <0.001
 daf-25(m98) STR-44 AWB 0 0 97 3 0 <0.001
  STR-163 AWB 47e 0 6 0 47 <0.001
  STR-163 ASK 0 0 35 30 35 <0.001
  SRBC-64 ASK 0 0 78 0 22 <0.001
 daf-25(m362) STR-44 AWB 0 0 100 0 0 <0.001

Adult animals grown at 20° were examined. n ≥ 20 animals. Expression from the same array was examined in wild-type and mutant strains.

a

Localization patterns were examined in animals exhibiting relatively wild-type ciliary morphology with the exception of unc-101 mutants. AWB and ASK cilia were either truncated or lacked a cilium in AWB in the majority of animals in unc-101 mutants (Dwyer et al. 2001; Kaplan et al. 2010).

b

Mutant strains were examined together with wild type in each experiment.

c

Differences among proportions in different categories were compared with respective wild-type values in the relevant neuron type for statistical significance. P-values were determined using a χ2 test of independence. Only differences at P < 0.05 or lower are indicated.

d

Cilia in these strains were visualized via expression of str-1p::mCherry (AWB) or srbc-66p::mCherry (ASK).

e

Expression was weak.