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. 2014 Mar 18;197(2):667–684. doi: 10.1534/genetics.114.161349

Table 4. Transition zone and IFT components required for trafficking and localization of GPCR::GFP fusion proteins to AWB and ASK cilia.

Strainb Fusion protein Neuron % localized to:a
Cilium Cilium, PCMC/distal dendrite PCMC/Distal dendritec Cilium, dendrite, cell body Cell body No expression P-valued
Wild type STR-44 AWB 100 0 0 0 0 0
STR-163 AWB 100 0 0 0 0 0
STR-163 ASK 68 0 0 0 11 21
SRBC-64 ASK 0 95 0 0 0 5
TZ + inversin compartment
 mks-1(tm2705); mksr-2(tm2452); mksr-1(ok2092) STR-44 AWB 100 0 0 0 0 0
  STR-163 AWB 100 0 0 0 0 0
  STR-163 ASK 48 0 0 0 19 33
 nphp-4(tm925) STR-44 AWB 100 0 0 0 0 0
  STR-163 AWB 100 0 0 0 0 0
  STR-163 ASK 85 0 0 0 0 15
 mks-5(tm3100) STR-44e AWB 0 100 0 0 0 0 <0.001
  STR-163e AWB 0 100 0 0 0 0 <0.001
  STR-163 ASK 52 0 0 0 15 33
 Ex[str-1p::mks-5] STR-44 AWB 65e 35 0 0 0 0 <0.001f
 nphp-2(gk653) STR-44 AWB 100 0 0 0 0 0
  STR-163 AWB 100 0 0 0 0 0
  STR-163 ASK 68 0 0 0 9 23
  SRBC-64 ASK 0 95 0 0 0 5
IFT related
 osm-3(p802) STR-44 AWB 100 0 0 0 0 0
  STR-163 AWB 100 0 0 0 0 0
  STR-163 ASK 72 0 0 0 14 14
 kap-1(ok676) STR-44 AWB 100 0 0 0 0 0
  STR-163 AWB 100 0 0 0 0 0
  STR-163 ASK 67 0 0 0 14 19
 tub-1(nr2004) STR-44e AWB 0 100 0 0 0 0 <0.001
  STR-163e AWB 0 100 0 0 0 0 <0.001
  STR-163e ASK 0 0 81 0 0 19 <0.001
  SRBC-64 ASK 0 100 0 0 0 0
 tub-1(nr2044) STR-163e AWB 0 100 0 0 0 0 <0.001
 daf-10(e1387) STR-44e AWB 0 100 0 0 0 0 <0.001
  STR-163e AWB 0 100 0 0 0 0 <0.001
  STR-163 ASK 62 0 0 0 24 14
  SRBC-64 ASK 0 0 100 0 0 0 <0.001
 daf-10(p821) STR-44e AWB 0 100 0 0 0 0 <0.001
SRBC-64 ASK 0 0 100 0 0 0 <0.001

Adult animals grown at 20° were examined. n ≥ 20 animals each. Expression from the same array was examined in wild-type and mutant strains except for the mks-1; mksr-2; mksr-1 triple mutant strain, which was injected with the indicated fusion constructs. TZ, transition zone.

a

Localization patterns were examined in animals exhibiting relatively wild-type ciliary morphology with the exception of mks-5, tub-1, daf-10, and osm-3 mutants. AWB cilia are truncated in mks-5 and tub-1 mutants; AWB and ASK cilia are truncated in daf-10 mutants; ASK cilia are truncated in osm-3 mutants.

b

Mutant strains were examined together with wild type in each experiment.

c

Weak expression was observed in cilia.

d

Differences among proportions in different categories were compared with respective wild-type values unless indicated otherwise in the relevant neuron type for statistical significance. P-values were determined using a χ2 test of independence. Only differences at P < 0.05 or lower are indicated.

e

Cilia in these strains were visualized via expression of str-1p::mCherry (AWB) or srbc-66p::che-13::TagRFP (ASK).

f

As compared to values in mks-5 mutants. The AWB ciliary morphological defects were also rescued (see Figure 4B).