Skip to main content
. 2014 Apr 1;197(2):701–714. doi: 10.1534/genetics.114.164574

Figure 2.

Figure 2

Comparison of the gene order at the vic1-1 and vic1-2 alleles. The sequences at the two alleles were BLAST-aligned in WebACT (http://www.webact.org/WebACT/home) for visualization of the genetic organization at each allele relative to that at the other with the Artemis Comparison Tool. High-scoring alignments are visualized in red (forward matches) and blue (reverse matches); the cutoff for an alignment was set at an ID score of ≥16. The vic1 locus contains a region that has undergone a sequence inversion affecting the orientation of a putative clavaminate synthatase-like gene relative to that of the predicted HET gene. Allele 1 is indicated below the alignment drawn to approximate scale. Note that allele 1 also contains a ∼8.5-kb insertion that is absent in allele 2. The allele-1-specific sequence contained two ORFs encoding a 689-aa HET-domain-containing protein (designated vic1d-1) and a 1619-aa TY1/copia-like LTR retrotransposon (designated vic1c-1) as indicated below the allele sequence alignment.