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. 2014 Jun 19;9(6):e100449. doi: 10.1371/journal.pone.0100449

Figure 2. Immunolocalization of Cthrc1 in various remodeling mouse tissues.

Figure 2

Immunohistochemistry was performed using rabbit monoclonal antibody Vli-55. (A) In response to angiotensin II, Cthrc1 is induced in interstitial myocardial cells of wildtype mice, but no expression is detected in similarly treated Cthrc1 null mice (B). (C) Angiotensin II-induced Cthrc1 in outer mural cells of the renal artery of wildtype mice (arrows) but not Cthrc1 null mice (D). (E) A skin incisional wound with Cthrc1 localization in activated dermal cells but not in the epidermis (e). (F) Ischemic muscle injury led to Cthrc1 protein expression in interstitial cells but not myocytes (m). (G) An arthritic joint with Cthrc1 in activated synovial fibroblasts (sf) but not in chondrocytes (c) is shown. (H) Remodeling tumor vessels of a germ cell tumor reveal Cthrc1 in outer mural cells (arrows), and (I) pancreatic ductal cancer shows Cthrc1 in cells of the surrounding stroma (s) but not in the tumor cells (t). Cthrc1 immunoreactivity in brown with hematoxylin counterstain. Original magnification 100x (C), 200x (B, D, E, G, I), and 400x (A, F, H).