Figure 3. Cthrc1 localizes to tumor stroma.

Immunolocalization of Cthrc1 was performed in various human cancers to determine protein levels in stromal cells (S) and tumor cells (T). (A) Melanoma biopsies showed few Cthrc1-positive stromal cells surrounding the tumor (red stain, arrows). (B) Endometrial cancer had abundant Cthrc1 positive stroma adjacent to necrotic tumor area. (C) The endometrial cancer was stained with an irrelevant rabbit monoclonal antibody to ensure specificity. Tumor stroma from (D) breast ductal carcinoma, (E) colon adenocarcinoma, (F) pancreatic adenocarcinoma, and (G, H) lung carcinoma had abundant Cthrc1 protein localization surrounding the tumor cells. (I) Some adipocytes adjacent to the tumor had Cthrc1 expression (arrows). Cthrc1 immunoreactivity in brown (except A) with hematoxylin counterstain. Original magnification 200x.