Table 2.
Proximal tibia trabecular bone morphology and remodeling
| Normal |
CKD |
One-way ANOVA |
|||||
|---|---|---|---|---|---|---|---|
| Vehicle | ZOL 100 µg | ANOVA | ZOL 20 µg | ZOL 100 µg | Calcium | ||
| BV/TV (%) | 14.5±1.4 | 18.6±1.2 | 13.9±1.8 | 24.1±1.6#* | 27.5±5.4#* | 24.9±1.0#* | 0.0002 |
| Tb.N (#/mm) | 1.54±0.12 | 2.08±0.09 * | 1.41±0.18 | 2.52±0.16 #* | 2.77±0.44 #* | 2.49±0.08 #* | <0.0001 |
| Tb.Th (µm) | 93 ±3 | 89±3 | 99±2 | 96±1 | 97±6 | 100±2 | 0.14 |
| Single label in animal, no. of animals | 8/8 | 7/7 | 6/6 | 7/7 | 5/5 | 6/6 | |
| Single label surface/bone surface (%) | 23.5±1.5 | 5.3±1.2# | 21.2±2.2 | 13.8±3.0#* | 8.4±2.1#* | 16.4±1.5# | <0.0001 |
| Double label in animal, # of animalsa | 7/8 | 3/7 | 5/6 | 4/7 | 5/5 | 4/6 | |
| Single label in ROI, # of animals | 7/7 | 3/3 | 5/5 | 4/4 | 5/5 | 4/4 | |
| Double label in ROI, # of animals | 7/7 | 0/3 | 5/5 | 0/4 | 2/6 | 4/4 | |
| MAR (µm/day) | 1.47±0.07 | – | 2.28±0.25# | – | 0.66±0.13#* | 1.19±0.12* | 0.0002 |
| BFR/BS (µm3/µm2/year) | 38.3±4.5 | – | 88±12# | – | 4.29±1.1#* | 14.1±2.4#* | <0.0001 |
| Osteoid thickness (µm) | 8.9±0.5 | 7.8±0.6 * | 9.5±0.4 | 7.0±0.5 #* | 7.3±0.5#* | 6.6±0.4 #* | 0.0010 |
| Osteoclast surface/bone surface (%) | 0.57±0.23 | 2.00±0.72 | 1.42±0.54 | 3.83±0.87 #* | 3.30±1.22# | 0.54±0.32 | 0.007 |
Data presented as mean ± SE
BV/TV bone volume per tissue volume, Tb.N trabecular number, Tb.Th trabecular thickness, MAR mineral apposition rate, MS/BS mineralizing surface per bone surface, BFR/BS bone formation rate per bone surface
a
Double labels were sought throughout three sections of the proximal tibia, both within and outside the measurement region of interest to confirm true administration of both labels, as well as in three sections of the cortical diaphysis on both periosteal and endocortical surfaces
#
p<0.05 versus normal vehicle group
*
p<0.05 versus CKD vehicle