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. 2014 Jun 19;9(6):e100621. doi: 10.1371/journal.pone.0100621

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© 2014 Molenda et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) Calculation of flux per TJ length revealed a significant lower value for 16HBE14o^– cells under resting conditions (red box) compared to CFBE41o^– cells (green box). Stimulation with cAMP caused a 3 fold increase for 16HBE14o^– cells (red hatched box) while CFBE41o^– cells do not respond to stimulation (green hatched box). B) CFBE-WT cells (blue) and CFBE-delF (brown) do not show statistically significant differences in fluorescein flux per TJ length neither under resting conditions nor upon stimulation with cAMP (hatched boxes). Data are presented as a box-plot showing raw data (circles), median (horizontal line) 25 and 75 percentile (box) and SD (whiskers (n = 7–13, p<0.05).