Figure 2. Relative fitness distribution and morphology of C. glabrata gene deletion strains.

(a) Wild type and mutant strains were grown in rich medium at 30°C and doubling times were recorded. The median doubling time of the wild type C. glabrata ATCC2001 strain under these conditions was 63.9 min while the median for mutant strains was 68.1 min. For each strain, the relative fitness was calculated using doubling times from at least two independent cultures of 1–6 independent mutants. Strains that showed strong variations in relative fitness between independent mutants or independent cultures were omitted from further analysis (Table S5). Data were obtained for 503 knock-out mutants (Table S6). (b) Colony and cell morphologies of C. glabrata deletion strains. Different types of distinct cell and colony morphologies were found. Cell morphology classes: ellipsoid (a), chains (b, f, g, i, k, l), elongated (c, d), large clumps (d, j, m, n), round (e), large and round (h); colony morphologies: smooth (a, b, c, e, f, g, i), small (h), slightly wrinkled (k, l), wrinkled (d, j, m, n). WT = HTL background strain; white bars correspond to 10 µm.