Figure 5. Clustering of susceptibility data of the C. glabrata gene deletion collection.
The set of mutants was screened on plates for hypersensitivity to four distinct stress conditions (CF, CR, CW and 42°C heat stress) using serial dilution assays on agar plates. (A) Two-dimensional hierarchical cluster plot of chemical-genetic profiles. On the horizontal axis genes are listed and stress conditions on the vertical axis. Interactions are shown in yellow depending on the degree of growth sensitivity (yellow = no growth, black = WT growth). Stress conditions and genes are clustered by the similarity of their interactions. (B) The clusters of genes are enlarged to highlight the chemical-genetic interaction profile of Caspofungin (blue bar in ‘a’ labeled ‘b’). (C) A section of the cluster (orange bar labeled ‘C’) involved in heat stress (42°C) is enlarged. (D) Genes implicated in sensitivity to the cell wall-perturbing agents CR and CW (red and green bar labeled ‘D’).
